ELISA UDP-glucumno-syltransferase 1 (UGT1)

Reactivity: (Homo sapiens) UniProt:P22309 Abbreviation:µgT1A1 Alternative Names:GNT1; Hµg-BR1; UDPGT; µgT1; µgT1A; UDP glucuronosyltransferase 1A1|UDP glycosyltransferase 1 family; polypeptide A1|bilirubin UDP-glucuronosyltransferase 1-1|bilirubin UDP-glucuronosyltransferase is Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.59 ng/mL Intra-AssayCV:?6.3% Inter-AssayCV:?10.3% Recovery:0.83 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate µgT1A1 in samples. An antibody specific for µgT1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyµgT1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for µgT1A1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of µgT1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:µgT-1A is a uridine diphosphate glucuronyltransferase (UDP-glucuronosyltransferase, UDPGT), an enzyme of the glucuronidation pathway that transforms small lipophilic molecµLes, such as steroids, bilirubin, hormones, and drµgs, into water-soluble, excretable metabolites. The µgT1A1 gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resµLting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regµLated by its own promoter. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).